Steroid Analysis

Analytical procedure

Solid phase extraction, hydrolysis, derivatization, gel filtration, gas chromatography-mass spectrometry (GC-MS)

Quantitative analysis  
a) Clinical routine: 41 metabolites (glucocorticoids, mineralocorticoids, progesterones,
estrogens, androgens) including steroid ratios for detection of
disorders in steroid biosynthesis or metabolism (steroid profile)

Urinary free (unconjugated) cortisol/cortisone (UFF/UFE)
   
b) Research: 64 metabolites (glucocorticoids, mineralocorticoids, progesterones,
estrogens, androgens)

Urinary free (unconjugated) cortisol/cortisone (UFF/UFE)

Bile acids (upon request)

Cholesterol metabolites (upon request)

Glycyrrhetinic acid (upon request)

Sample

Clinical routine: Urine (30 ml of a 24h urine preferred, please indicate 24h volume; spot sample
possible)
Research: Urine (30 ml of a 24h urine preferred, please indicate 24h volume; spot sample
possible)
Plasma, serum, liquor, amniotic fluid, dialysate, cell culture supernatants
(5 to 10 ml each)
Cell and tissue extracts (upon request)

Costs

Quantitative analysis for diagnostic purposes (steroid profile): CHF 200.–
(In Switzerland paid by health insurance; samples from abroad: direct payment)

Analyses of samples with scientific background upon request:
Prof. Dr. med. Bruno Vogt, Dr. med. Daniel Ackermann

Forms

  • English
  • German




Information

Scientific and clinical questions
Dr. med. Daniel Ackermann
Department of Nephrology and Hypertension
University Hospital / Inselspital
Freiburgstrasse 15
CH-3010 Berne
Switzerland
Phone +41 31 632 9629
Fax +41 31 632 4436
E-mail daniel.ackermann@no-spaminsel.ch

Analytical and technical questions
Dr. pharm. Bernhard Dick
Department of Nephrology and Hypertension
University Hospital / Inselspital
Freiburgstrasse 15
CH-3010 Berne
Switzerland
Phone +41 31 632 9289
Fax +41 31 632 9444
E-mail bernhard.dick@no-spamdkf.unibe.ch