Steroid Analysis

Mission

The main tasks of the Laboratory for Steroid Analysis are the following:

  • Routine analysis of clinical samples (urine)
  • Research collaborations (analysis of steroids in other biological matrices such as e.g. serum, cells and tissue extracts)
  • Development of novel analytical methods for steroid analysis

Analytical procedure

All analyses are performed using mass spectrometric techniques. The procedure used for routine analysis comprises the following steps:

  • Steroid extraction (solid phase or liquid/liquid)
  • Hydrolysis
  • Derivatisation
  • Sample clean-Up
  • Gas chromatography-mass spectrometry(GC-MS)

For research project we also have a high-resolution GCxGC-TOFMS platform to our disposal. 

Additionally, LC-MS/MS based analysis is offered in collaboration with the Clinical Metabolomics Facility of Department of Clinical Chemistry.

Routine Analysis
  • 41 urinary steroid metabolites (quantitative)
  • Quantification of urinary free cortisol/cortisone
  • exogenous steroids

Click here for a complete list of measured analytes.

Sample Material

  • Urine: at least 5 ml  of a 24h urine collection (preferred) or spot urine
    No convervatives or cooling required. Analysis of acidified urine possible. Please attached completely filled in sample submission form.
  • Plasma, Serum, cell and tissue extract on request

Costs

Please inquire.

Information

General and technical information

PD Dr.  Michael Grössl
Tel. +41 31 632 9289
E-Mail michael.groessl@no-spamdbmr.unibe.ch

 

Clincial information

Dr. med. Daniel Ackermann
Tel. +41 31 632 9629
E-Mail Daniel.Ackermann@no-spaminsel.ch